The Impact of Varied Levels and Application Methods of Boric Acid on the Performance and Protein Content

Fakher Kardoni *

Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

Somayeh Karami

Faculty of Agriculture and Natural Resources, University of Ramin, Ahvaz, Iran.

Farzad Jame Andarzgoo

Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

Sana Dashti

Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

*Author to whom correspondence should be addressed.


This research investigates the influence of boron (B) application on the yield and protein content of common bean (Phaseolus vulgaris L.), a crop of significant importance due to its high protein and essential amino acid content. The study underscores boron's crucial, albeit still unclear, role in plant growth, and notes the damage boron deficiency and excess can cause, such as thickened stems, rough leaf tissues, stunted growth, and leaf scorching. Highlighting the plant species-dependent variance in optimal boron requirements, with legumes including common bean often needing more, the study reveals that a foliar application of 0.05% boric acid concentration, applied twice post-emergence, maximizes seed yield, while higher concentrations are detrimental. The research thus emphasizes the necessity of striking a balance in boron application to avoid either deficiency or toxicity, offering insights into appropriate boron dosage and application method for effective common bean cultivation. By contributing to a broader understanding of boron's role in common bean production and offering directions for optimized fertilization practices, this study helps improve both crop yield and quality. However, it also signals the need for further exploration of the long-term effects of boron application and its interaction with other nutrients under different environmental conditions.

Keywords: Boron, crop production, legume, yield potential

How to Cite

Kardoni , Fakher, Somayeh Karami, Farzad Jame Andarzgoo, and Sana Dashti. 2023. “The Impact of Varied Levels and Application Methods of Boric Acid on the Performance and Protein Content”. Asian Journal of Research in Crop Science 8 (4):381-91.


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Shimelis EA, Rakshit SK. Proximate composition and physico-chemical properties of improved dry bean (Phaseolus vulgaris L.) varieties grown in Ethiopia. LWT-Food Sci. Technol. 2005; 38:331–338.

Hayat I, Ahmad A, Masud T, Ahmed A, Bashir S. Nutritional and health perspectives of beans (Phaseolus vulgaris L.): An overview. Crit. Rev. Food Sci. Nutr. 2014;54:580–592. DOI: 10.1080/10408398.2011.596639

Stirk WA, Bálint P, Tarkowská D, Strnad M, van Staden J, Ördög V. Endogenous brassinosteroids in microalgae exposed to salt and low temperature stress. Eur. J. Phycol; 2018.

Van Oosten MJ, Pepe O, De Pascale S, Silletti S, Maggio A. The role of biostimulants and bioeffectors as alleviators of abiotic stress in crop plants. Technol. Agric. 2017;4:5.

Drobek M, FR AC, M, Cybulska J. Plant biostimulants: Importance of the quality and yield of horticultural crops and the improvement of plant tolerance to abiotic stress—A review. Agronomy. 2019;9: 335.

Chanthini KP, Stanley-Raja V, Thanigaivel A, Karthi S, Palanikani R, Shyam Sundar N, Sivanesh H, Soranam R, Senthil-Nathan S. Sustainable agronomic strategies for enhancing the yield and nutritional quality of wild tomato, Solanum lycopersicum (l) var cerasiforme Mill. Agronomy. 2019;9:311.

Mirbakhsh M. Role of Nano-fertilizer in Plants Nutrient Use Efficiency (NUE). J Gene Engg Bio Res. 2023;5(1):75-81.

Rengasamy KRR, Kulkarni MG, Stirk WA, Van Staden, J. Eckol—A new plant growth stimulant from the brown seaweed Ecklonia maxima. J. Appl. Phycol. 2015; 27:581–587.

Marquezi M, Gervin VM, Watanabe LB, Moresco R, Amante ER. Chemical and functional properties of different common Brazilian bean (Phaseolus vulgaris L.) cultivars. Braz. J. Food Technol. 2017;20: e2016006. DOI: 10.1590/1981-6723.0616

Mirbakhsh M, Zahed Z. Enhancing phosphorus uptake in sugarcane: A critical evaluation of humic acid and phosphorus fertilizers’ effectiveness. J Gene Engg Bio Res. 2023;5(3):133-145.

Mirbakhsh M, Sohrabi Sedeh SS, Zahed Z. The impact of Persian clover (Trifolium resupinatum L.) on soil health. BSJ Agri. 2023;6(5):564-570.

Mirbakhsh M, Sohrabi Sedeh SS, Zahed Z. The impact of persian clover (Trifolium resupinatum L.) on soil health. Black Sea Journal of Agriculture. 2023;6(5):564-570. DOI: 10.47115/bsagriculture.1312940

Gupta UC. Boron deficiency and toxicity symptoms for several crops as related to tissue boron levels. Journal of Plant Nutrition. 1983;6:387-395.

Acosta-Gallegos JA, Kelly JD, Gepts P. Prebreeding in common bean and use of genetic diversity from wild germplasm. Crop Sci. 2007;47:S-44–S-59. DOI: 10.2135/cropsci2007.04.0008IPBS

Hall R, HF. Schwartz. Common beans. In: Willam F. Bennett (ed.), Nutrient deficiencies and toxicities in crop plants, Minnesota, ASP Press. 1994;143-148.

Luthria DL, Pastor-Corrales MA. Phenolic acids content of fifteen dry edible bean (Phaseolus vulgaris L.) varieties. J. Food Compos. Anal. 2006;19:205–211. DOI: 10.1016/j.jfca.2005.09.003

Bitocchi E, Rau D, Bellucci E, Rodriguez M, Murgia ML, Gioia T, Santo D, Nanni L, Attene G., Papa R. Beans (Phaseolus ssp.) as a model for understanding crop evolution. Front. Plant Sci. 2017;8:722. DOI: 10.3389/fpls.2017.00722

Trautmann RR, Lana MC, Guimarães VF, Junior ACG, Steiner F. Soil water potential and boronfertilization in growth and uptake of the nutrient for the soybean crop. Revista Brasileira De Ciência Do Solo. 2014;38:240–51.

Lemiska A, Pauletti V, Cuquel FL, Zawadneak MAC. Production and fruit quality of strawberry under boron influence. Cienc. Rural. 2014;44: 622-628.

Yadegari M. Effect of micronutrients foliar application and biofertilizeres on essential oils of lemon balm. J. Soil Sci. Plant Nutr. 2016;16:702-715.

Zhu H, Zhao Y, Nan F, Duan Y, Bi R.. Relative influence of soil chemistry and topography on soil available micronutrients by structural equation modeling. J. Soil Sci. Plant Nutr. 2016;16:1038-1051.

Tiffen LO. Translocation of micronutrients in plants. Soil Sci. Soc. Of America, Madison. USA. 1972;199-229.

Choung MG. Choi BR, An YN, Chu YH, Cho YS. Anthocyanin profile of Korean cultivated kidney bean (Phaseolus vulgaris L.) J. Agric. Food Chem. 2003; 51:7040–7043. DOI: 10.1021/jf0304021

Silva DH, Boaretto AE. Boron mobility in Castro bean plant. The Proceeding of International Plant Nutrition Colloquium XV1; 2009.

Yang QQ, Gan RY, Ge YY, Zhang D, Corke H. Polyphenols in common beans (Phaseolus vulgaris L.): Chemistry, analysis, and factors affecting composition. Compr. Rev. Food Sci. Food Saf. 2018;17: 1518–1539.

DOI: 10.1111/1541-4337.12391

Chen PX, Tang Y, Marcone MF, Pauls PK, Zhang B, Liu R, Tsao R. Characterization of free, conjugated and bound phenolics and lipophilic antioxidants in regular-and non-darkening cranberry beans (Phaseolus vulgaris L.) Food Chem. 2015; 185:298–308. DOI: 10.1016/j.foodchem.2015.03.100

Teixeira-Guedes CI, Oppolzer D, Barros AI, Pereira-Wilson C. Impact of cooking method on phenolic composition and antioxidant potential of four varieties of Phaseolus vulgaris L. and Glycine max L. LWT. 2019;103:238–246. DOI: 10.1016/j.lwt.2019.01.010

Martens DC, Westermann DT. Fertilizer application for correcting micronutrient deficiencies. In: Mortvedt JJ, et al. (eds.) Micronutrients in Agriculture. Second Ed. SSSA Madison. USA. 1991;549-592.

Castr J, Sotomayor C. The influence of boron and zinc sprays bloom time on almond fruit set. Acta- Hort. 1997; 402-405.

Nasef MA, Badran NM, Abd El- Hamide AF. Response of peanut to foliar spray with boron and/or rhizobium inoculation. Journal of Applied Sciences Research. 2006;2(12):1330-1337.

Shabaan MM, El-Fouly MM, Abou El-Nour EAA. Boron/nitrogen interaction effect on growth and yield of faba bean plants grown under sandy soil condition. International Journal of Agricultureal Research. 2006; 1(4):322-330.

Di Mola I, Cozzolino E, Ottaiano L, Giordano M, Rouphael Y, Colla G, Mori M. Effect of vegetal- and seaweed extract-based biostimulants on agronomical and leaf quality traits of plastic tunnel-grown baby lettuce under four regimes of nitrogen fertilization. Agronomy. 2019;9:571.

Guptu UC, YA. Cutcliffe. Effect of applied and residual boron on the nutrition of cabbage and field beans. Can. Journal Soil Science. 1984;64:571-576.

Madrera RR, Valles BS. Development and validation of ultrasound assisted extraction (UAE) and HPLC-DAD method for determination of polyphenols in dry beans (Phaseolus vulgaris) J. Food Compos. Anal. 2020;85:103334.

DOI: 10.1016/j.jfca.2019.103334

Mirbakhsh M, Brouder SM, Volenec JJ. Temporal Change of Carbon Stocks and Soil Health Indicators on a Long-Term Experimental Site: Annual Vs. Perennial Systems [Abstract]. ASA, CSSA, SSSA International Annual Meeting, Baltimore, MD;2022. Available: