Evaluation of Upland Rice Response to Water Stress Using Polyethylene Glycol (PEG -6000) at Germination and Early Seedling Stage

Emmanuel Momolu Pope *

Department of Seed, Crop and Horticultural Sciences, University of Eldoret, Kenya.

Wilson Opile

Department of Seed, Crop and Horticultural Sciences, University of Eldoret, Kenya.

Lucas Ngode

Department of Seed, Crop and Horticultural Sciences, University of Eldoret, Kenya.

Emmy Chepkoech

Department Biotechnology, University of Eldoret, Kenya.

*Author to whom correspondence should be addressed.


Abstract

Water stress is one of the most important crop growth limiting factors which leads to low crop productivity and yield instability. Water stress affects crop growth and development, especially during the germination and seedling period. The experiment was conducted in the growth chamber at the seed physiology laboratory of the Department of Seed, Crop and Horticultural Sciences, University of Eldoret to evaluate sixteen upland rice varieties to water stress tolerance at germination and early seedling growth stage. These varieties were tested against four levels of water stress imposed by Polyethylene glycol 6000 (PEG - 6000) at 0%, 5%,10% and 15% concentration. The lay out of the experiment was complete randomized design (CRD) with three replications. Osmotic stress induced by PEG levels significantly (P<.001) reduced plant growth parameters. The result showed that the germination percentage, germination index, Relative seedling height (%), seedling dry weight, Seedling fresh weight, Seedling length, seedling vigor index and mean germination time of all tested rice varieties were found decreasing trends with increasing the levels of PEG from 0 to 15% concentration levels. Among all the sixteen varieties, NERICA rice varieties followed by MWUR, White rice, Kpatawee, and Komboka had an outstanding performed in terms of germination percentage, germination index, seedling height, seedling dry weight, root length and relative dry weight under water induced stress by using PEG concentration levels compared to other varieties. Therefore, these varieties could be useful in breeding programs and can be cultivated in arid and semi-arid environment or where water shortage is a regular constraint.

Keywords: Water stress, upland rice response, polyethylene glycol 6000, germination and early seedling stage


How to Cite

Pope , E. M., Opile , W., Ngode , L., & Chepkoech , E. (2024). Evaluation of Upland Rice Response to Water Stress Using Polyethylene Glycol (PEG -6000) at Germination and Early Seedling Stage. Asian Journal of Research in Crop Science, 9(1), 37–49. https://doi.org/10.9734/ajrcs/2024/v9i1244

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References

Rajput GS, Kuruwanshi VB, Guhey A. Polyethylene glycol induced screening for drought tolerance of different rice genotype. The Pharma Innovation Journal. 2022;11(9):996-1000. ISSN (E): 2277-7695 ISSN (P): 2349-8242.

Khan A, Shen F, Lixue Y, Xing W, Clothier B. Limited Acclimation in Leaf Morphology and Anatomy to Experimental Drought in Temperate Forest Species. Biology. 2022;11:1186. Available:https://doi.org/10.3390/ biology11081186.

Fukagawa NK, Ziska LH. Rice: importance for global nutrition. Journal of Nutritional Science. Vitamin. 2019;65:S2–S3. DOI: 10.3177/jnsv.65. s2.

Khodarahmpour Z. Effect of drought stress induced by polyethylene glycol (PEG) on germination indices in corn (Zea mays L.) hybrids. African Journal of Biotechnology. 2011;10(79):18222-18227.

Mostajeran A, Rahimi-Eichi V. Effects of Drought Stress on Growth and Yield of Rice (Oryza sativa L.) Cultivars and Accumulation of Proline and Soluble Sugars in Sheath and Blades of Their Different Ages Leaves. American-Eurasian J. Agric. & Environ. Sci. 2009;5(2):264-272. ISSN 1818-6769.

Yousefi AR, Rashidi S, Moradi P, Mastinu A. Germination and Seedling Growth Responses of Zygophyllum fabago, Salsola kali L. and Atriplex canescens to PEG-Induced Drought Stress. Environments. 2020;7(12):107.

Ahmad S, Ahmad R, Ashraf MY, Ashraf M. Waraich EA. Sunflower (Helianthus Annuus L.) response to drought stress at germination and seedling growth stages. Pak J Bot. 2009;41:647-54.

Sokoto MB, Muhammad A. Response of rice varieties to water stress in Sokoto, Sudan Savannah, Nigeria. Journal of Bioscience Med. 2014;2:68–74.

Shamim F, Saqlan SM, Athar H-u-R, Waheed A. Screening and selection of tomato genotypes/cultivars for drought tolerance using multivariate analysis. Pakistan Journal of Botany. 2014;46:1165-1178.

Bagher G, Ghorbani M, Ghasemi M. Effects of different levels of osmotic potential on germination percentage and germination rate of barley, corn and canole. Iranian Journal of Plant Physiology. 2012;2:413-417.

Hussain HA, Hussain S, Khaliq A, Ashraf U. Anjum SA. Men S, Wang L. Chilling and Drought Stresses in Crop Plants: Implications, Cross Talk, and Potential Management Opportunities. Front. Plant Sci. 2018;9:393. DOI: 10.3389/fpls.2018.00393.

Swamy BPM, Kumar A. Sustainable rice yield in water-short drought-prone environments: Conventional and molecular approaches. In: Lee T S. Irrigation Systems and Practices in Challenging Environments. German; 2012.

Xie X. Zhang X, He Q. Identification of drought resistance of rapeseed (Brassica napus L.) during germination stage under PEG stress. Journal of Food, Agriculture and Environment. 2013;11(2):751-756.

Swapna S, Shylaraj KS. Screening for Osmotic Stress Responses in Rice Varieties under Drought Condition. Science Direct Rice Science. 2017;24(5):253-263.

Gairola KC. Nautiyal AR, Dwivedi AK. Effect of Temperatures and Germination Media on Seed Germination of Jatropha Curcas Linn. Advances in Bioresearch. 2011;2(2):66–71. ISSN 0976-4585.

Swain P, Anumalla M, Prusty S, Marndi BC, Rao GJN. Characterization of some Indian native land race rice accessions for drought tolerance at seedling stage. Australian Journal of Crop Science. 2014;8(3):324–331.

Wang C, Zhou L, Zhang G, Xu Y, Gao X, Jiang N, Zhang L, Shao M. Effects of Drought Stress Simulated by Polyethylene Glycol on Seed Germination, Root and Seedling Growth, and Seedling Antioxidant Characteristics in Job’s Tears. Agricultural Sciences. 2018;9:991-1006.

Sagar A, Rauf F, Mia M, Shabi T, Rahman T, Hossain A. Polyethylene glycol (PEG) induced drought stress on five rice genotypes at early seedling stage. Journal of Bangladesh Agricultural University. 2020;18(3):606–614.

Basu S, Roychoudhury A, Saha PP, Sengupta DN. Comparative Analysis of Some Biochemical Responses of Three Indica Rice Varieties during Polyethylene Glycol-Mediated Water Stress Exhibits Distinct Varietal Differences. Acta Physiologiae Plantarum. 2010;32:551-563.

Ahmed S, Ullah Z, ul Haq Z, Muhammad, Raza Siddiqui MZ. Latif, S. Effect of moisture stress on seed germination and early seedling growth of pulse crops. Pure and Applied Biology. 2018;7(2):775-782.

Shinohara T, Sanada A, Terada N, Ron L, Koshio K.. Seed Germination and Seedling Growth of Yellow and Purple Passion Fruit Genotypes Cultivated in Ecuador.Horticulturae. 2022;8:754. Available:Phttps://doi.org/ 10.3390/horticulturae8080754

Bareke T. Biology of seed development and germination physiology Adv. Plants Agric. Res. 2018;8(4):336‒46.

Roy RC, Sagar A, Tajkia JE, Razzak MA, Hossain AKMZ. Effect of salt stress on growth of sorghum germplasms at vegetative stage. Journal of the Bangladesh Agricultural University. 2018;16:67–72.

Islam MM, Kayesh EZ, aman ET, Urmi A, Haque MM. Evaluation of Rice (Oryza sativa L.) Genotypes for Drought Tolerance at Germination and Early Seedling Stage.TheAgriculturists 16(1): 44-54 (2018). A Scientific Journal of Krishi Foundation; 2018. ISSN 2304-7321 (Online), ISSN 1729-5211.

Liu K, Muse SV. PowerMarker: Integrated Analysis Environment for Genetic Marker Data. Bioinformatics. 2015;21:2128-2129.

Kouighat M, Hanine H, El Fechtali M, Nabloussi A. First Report of Sesame Mutants Tolerant to Severe Drought Stress during Germination and Early Seedling Growth Stages. Plants. 2021;10: 1166. Available:https://doi.org/10.3390/ plants10061166.

Zahedifar M, Zohrabi S. Germination and seedling characteristics of drought-stressed corn seed as influenced by seed priming with potassium nano-chelate and sulfate fertilizers. Acta Agriculturae Slovenica. 2016;107(1):113-128.

Shatpathy P, Kar M. Dwibedi SD, Dash, A. Seed Priming with Salicylic Acid Improves Germination and Seedling Growth of Rice (Oryza sativa L.) under PEG-6000 Induced Water Stress. International Journal of Current Microbiology and Applied Sciences. 2018;7(10):907-924.

Meneses CHG, Bruno RLA. Fernandes PD. Pereira WE, Lima LHM, Lima MMA, Vidal MS. Germination of cotton cultivar seeds under water stress induced by polyethyleneglycol-6000. Scientia Agricola. 2011;68(2):131-138.

Türkoglu A, Tosun M, Haliloğlu K, Karagöz H. Effects of Early Drought Stress on Germination and Seedling Growth Parameters of Kırik Bread Wheat (Triticum aestivum L.), Eregli Journal of Agricıultural Science. 2022;2(2):75-80.

Verslues PE. Agarwal M, Katiyar-Agarwal S, Zhu J, Zhu JK. 2007. Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J. 2006;45:523–539.

Yousefi AR, Rashidi S, Moradi P, Mastinu A. Germination and Seedling Growth Responses of Zygophyllum fabago, Salsola kali L. and Atriplex canescens to PEG-Induced Drought Stress. Environments. 2020;7(12):107.