Control of Sugarcane Smut Disease: Evaluation of the In vitro Fungitoxicity of Three Essential Oils

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Yao Kouadio Jacques-Edouard
Kouamé Konan Didier
Kouamé Koffi Gaston
Kassi Koffi Fernand Jean-Martial
N’guessan Aya Carine
Camara Brahima
Koné Daouda


The sugarcane smut disease caused by Sporisorium scitamineum occurs in all production areas. In order to contribute to the management of this disease, a study was carried out in vitro conditions. The aim of this study was to evaluate the antifungal efficacy and to determine the inhibitory concentration of the essentials oils of Ocimum gratissimum, Zingiber officinale and Cymbopogon citratus upon two strains B and Z of Sporisorium scitamineum. These oils were tested on mycelial growth of colonies, spores’ production and teliospores germination. The results showed a variable fungitoxic effect of the oils according to doses. Mycelial growth and spores production were totally inhibited (100%) from 1000 ppm by all the oils. The 90% inhibitory concentrations (IC90) obtained were 880.37 ppm, 847.83 ppm and 805.75 ppm for Ocimum gratissimum, Zingiber officinale and Cymbopogon citratus, respectively. The germination of teliospores was completely reduced by the three essential oils from 500 ppm upwards. Propiconazole had strong inhibitory effects at 400 ppm with a very low IC50 and the IC90 ranged from 305 ppm to 323.36 ppm. Moreover, at the level of the strains, no difference in resistance was observed These oils could provide a good means of controlling sugarcane smut.

Essentials oils, in vitro, Sporisorium scitamineum, Côte d'Ivoire, Sugarcane

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Jacques-Edouard, Y. K., Didier, K. K., Gaston, K. K., Jean-Martial, K. K. F., Carine, N. A., Brahima, C., & Daouda, K. (2020). Control of Sugarcane Smut Disease: Evaluation of the In vitro Fungitoxicity of Three Essential Oils. Asian Journal of Research in Crop Science, 5(2), 34-41.
Original Research Article


FAO, 2017. Principaux pays agricoles et alimentaires et producteurs. Classification des pays dans le monde, par produit. Division de la statistique de la FAO.(visté le12/12/2019) Available:

Macedo IC, Seabra JEA. et Silva JEAR, Greenhouse gases emissions in the production and use of ethanol from sugarcane in Brazil: The 2005/2006 averages and a prediction for 2020. Biomass Bioenergy. 2008;32:582-595.

Pipenbring M. Stoll, M. Oberwinkler F. The generic position of Ustilago maydis, Ustilago scitaminea and Ustilago esculenta (Ustilaginales). Mycol. Progress. 2002;1: 71-80.

Thaung MM. Rust, smuts and their allies in Burma. Austral, Mycol. 2005;24:29-46.

Singh N, Somai BM, Pillay D. Smut disease assesment by PCR and microscopy in inoculated tissue cultured sugarcane cultivars. Plant Sci. 2004;167: 987-994.

Santiago R, de Armas R, Legaz ME, Vicente C. Separation from Ustilago scitaminea of different elicitors which modify the pattern of phenolic accumulation in sugarcane leaves. J Plant Pathol. 2008;90:87-96.

Alexander KC, Ramakrishnan K. Infection of the bud, establishment in the host and production of whips in sugarcane smut (Ustilago scitaminea) of sugarcane. Proc Intern Soc Sugarcane Technol. 1980;17: 1452-1455.

ISO (International Sugar Organization). 2006. Sugar Year Book. International Sugar Organization, London. [Online] Cited on March 22, 2019.


Bhuiyan SA. Croft BJ. Cox MC. et Bade G. Some biological parameters of the sugarcane smut fungus, Ustilago scitaminea. Proc. Aust. Soc. Sugar Cane Technol. 2009;31:125-13.

Sanchez Rodriguez R, Pino Algora JA, Vallin Plous C, Pérez Rodruez ME, Iznaga Sosa, Malpartida Romero F. Action du fongicide naturel F20 contre la cercosporiose noire (Mycosphaerella fijiensis Morelet) chez le bananier plantain (AAB) et la bananier (AAA). INFOMUSA. 2002;11(1):14-16.

Oussou KR. Étude chimique et activités biologiques des huiles essentielles de sept plantes aromatiques de la pharmacopée ivoirienne. Thèse de Doctorat Unique. Laboratoire de chimie organique et biologique, UFR SSMT, Université de Cocody-Abidjan.241 Pathology. 2009;57: 181-188.

Camara B, Koné D, Kanko C, Anno A. et Aké S.. Activité antifongique des huiles essentielles de Ocimum gratissimum L., de Monodora myristica (Gaaertn) Dunal et de deux produits de synthèses (Impulse et Folicur) sur la croissance mycélienne et la production de spore in vitro de Deightoniella torulosa (Syd.) Ellis. Rev. Ivoire Sci. Technol. 2007;09:187-201.

Rhayour Khadija, Sidi Mohamed, Ben Abdellah Touria, Bouchikhi Mohamed, Ettayebi Adnane Remma, Improved Method for the Determination of Antimicrobial Activity of Essential Oils in Agar Medium in Journal of Essential Oil Research. 2011;5(2):179-18.

Santiago Rocío, Roberto de Armas, María Blanch, Carlos Vicente & María-Estrella Legaz In vitro effects of caffeic acid upon growth of the fungi Sporisorium scitamineum. Journal of Plant Interactions. 2010;5(3):233-240.

Kouamé KD, N’guessan AC, Kassi KF, Kouamé KG Yao KJ-E, Yobouet AA, Koné D. et Zouzou M.. In vitro Fungitoxicity of Four Essential Oils on Sugarcane Smut Sporisorium scitamineum Piep., in Côte d'Ivoire SARJNP. 2018;1(3):1-10.

Ohno T, Kita M, Yamaoka Y, Imamura S, Yamamoto T, Mitsufuji S, Kodama T, Kashima K, Imanishi J. Antimicrobial activity of essential oils against Helicobacter pylori. Helicobacter. 2003;8: 207–215.

Dutta BK, Karmakar S, Naglot A, Aich JC, Begam M, Anticandidial activity of some essential oils of a mega biodiversity hotspot in India. Mycoses. 2007;50:121–124.

Kouame Koffi Gaston, Kouassi Koffi Ii Nazaire, Kassi Fernand Martial, Bolou Bi Bolou Antoine, Tuo Seydou, Kanko Coffi, Koné Daouda. Antifungal activity of essential oils extracted from Monodora myristica (Gaertn), Ocimum gratissimum L. And Zingiber officinalis Roscoe on post-harvest anthracnose of mango fruit (Mangifera indica L.) Variety Kent In Côte D’Ivoire IJS 4-December. 2015;12.

Kassi Fernand Martial1, Badou OJ, Tonzibo ZF, Amari SZLG, Kone D. Action du fongicide naturel NECO contre la Mycosphaerella fijiensis Morelet chez le bananier plantain (AAB) en Côte d’Ivoire. Journal of Applied Biosciences. 2014;75: 6183– 6191.

Doumbouya Mohamed, Abo Kouabenan, Lepengue A. Nicaise, Camara Brahima, Kanko Koffi, Aidaradaouda, Kone Daouda. Activités comparées in vitro de deux fongicides de synthèse et de deux huiles essentielles, sur des champignons telluriques des cultures maraîchères en Côte d’Ivoire. Journal of Applied Biosciences. 2012;50:3520–3532.

Leu LS, Teng WS, Wang ZN. Culmicolous smut of sugarcane in Taiwan, Resistant trial. Taiwan. Sugar Experiment Station Research Report. 1978;74:37-45.

Hamilton-kemp, Archbold. Stimulation and inhibition of fungal pathogens of plants by natural valatile phytochemicals and their analogs. Current Topics in Hytochemistry. 2000;4:95-04.

Bhuiyan SA, Croft BJ, Tucker GR. New method of controlling sugarcane smut using flutriafol fungicide. Plant Dis. 2015; 99:1367-1373.

Stephen Nesnow: Conazoles and Cancer A Review, Consulting, Chapel Hill, NC, USA Chapter. 2016;10:22.

Dabrowski JM, Shadung JM. et Wepener V. Prioritizing agricultural pesticides used in South Africa based on their environmental mobility and potential human health effects. Environment International. 2014;62:31-40.